>> Checked back and it was 25 May my cycle ride when I foolishly had a
>> 160m ascent.
I remember similar issues in the early eighties when I first started riding long distance with a group. My bike at the time was adapted from an off the shelf 'racer' and although I'd fitted mudguards and a rack I'd only made limited adjustments to the gearing. I'd also yet to learn to make full use of lower gears and did the 'exercise' thing of pushing in second or third (of five). Ditchling Beacon and some hard climbs in the Cotswolds/Forest of Dean plus keeping up with fitter riders were the likely causes
It cleared up with abstinence from riding for a few weeks but was prone to return with first serious rides of a new year.
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Knees 
Knees 